The 2000–2001 Daytime Emmy Awards were presented on May 18, 2001, at New York City's Radio City Music Hall.
| Outstanding Drama Series: | As the World Turns (CBS) |
| Lead Actor in a Drama Series: | David Canary, All My Children (ABC) |
| Lead Actress in a Drama Series: | Martha Byrne, As the World Turns (CBS) |
| Supporting Actor in a Drama Series: | Michael E. Knight, All My Children (ABC) |
| Supporting Actress in a Drama Series: | Lesli Kay, As the World Turns (CBS) |
| Younger Actor in a Drama Series: | Justin Torkildsen, The Bold and the Beautiful (CBS) |
| Younger Actress in a Drama Series: | Adrienne Frantz, The Bold and the Beautiful (CBS) |
| Drama Series Writing Team: | As the World Turns (CBS) |
| Outstanding Children's Series: | Reading Rainbow (PBS) |
| Outstanding Children's Special (tie): | Run the Wild Fields (Showtime) and A Storm in Summer (Showtime) |
| Outstanding Children's Animated Program: | Arthur (PBS) |
| Outstanding Children's Animated Program (special class): | Batman Beyond (WB) |
| Performer in a Children's Series: | Levar Burton, Reading Rainbow (PBS) |
| Performer in a Children's Special: | Ossie Davis, Finding Buck McHenry (Showtime) |
| Performer in an Animated Program: | Nathan Lane, Disney's Teacher's Pet (ABC) |
| Outstanding Preschool Children's Series: | Sesame Street (PBS) |
| Outstanding Special Class Series: | AMC's Behind the Screen with John Burke (AMC) |
| Outstanding Special Class Special: | Barbra Streisand and AMC Present “Reel Models: The First Women of Film” (AMC) |
| Outstanding Game and Audience Participation Show: | Who Wants to Be a Millionaire (ABC) |
| Outstanding Game-Show Host: | Regis Philbin, Who Wants to Be a Millionaire (ABC) |
| Outstanding Talk Show: | The Rosie O'Donnell Show (syndicated) |
| Outstanding Talk-Show Host (tie): | Rosie O'Donnell, The Rosie O'Donnell Show (syndicated) and Regis Philbin, Live with Regis |
| Outstanding Service Show: | Martha Stewart Living (syndicated) |
| Outstanding Service-Show Host: | Julia Child & Jacques Pepin, Julia and Jacques Cooking at Home (PBS) |
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