The 1999–2000 Daytime Emmy Awards were presented on May 19, 2000, at New York City's Radio City Music Hall.
| Outstanding Drama Series: | General Hospital (ABC) |
| Lead Actor in a Drama Series: | Anthony Geary, General Hospital (ABC) |
| Lead Actress in a Drama Series: | Susan Flannery, The Bold and the Beautiful (CBS) |
| Supporting Actor in a Drama Series: | Shemar Moore, The Young and The Restless (ABC) |
| Supporting Actress in a Drama Series: | Sarah Brown, General Hospital (ABC) |
| Younger Actor in a Drama Series: | David Tom, The Young and The Restless (ABC) |
| Younger Actress in a Drama Series: | Camryn Grimes, The Young and the Restless (ABC) |
| Drama Series Writing Team: | The Young and the Restless (CBS) |
| Outstanding Children's Series: | Disney Presents: Bill Nye the Science Guy (syndicated) |
| Outstanding Children's Special: | Summer's End (SHO) |
| Outstanding Children's Animated Program: | Steven Spielberg Presents: Pinky, Elmyra and the Brain (WB) |
| Outstanding Children's Animated Program (special class): | Rolie Polie Olie (DIS) |
| Performer in a Children's Series: | Shari Lewis, Charlie Horse Music Pizza (PBS) |
| Performer in a Children's Special: | James Earl Jones, Summer's End (SHO) |
| Performer in an Animated Program: | James Woods, Disney's Hercules (ABC) |
| Outstanding Preschool Children's Series: | Sesame Street (PBS) |
| Special Class Program: | 1999 Macy's Thanksgiving Day Parade (NBC) |
| Outstanding Game Show: | Who Wants to Be a Millionaire (ABC) |
| Outstanding Game-Show Host: | Bob Barker, The Price Is Right (CBS) |
| Outstanding Talk Show: | The Rosie O'Donnell Show (syndicated) |
| Outstanding Talk-Show Host: | Rosie O'Donnell, The Rosie O'Donnell Show (syndicated) |
| Outstanding Service Show: | Martha Stewart Living (syndicated) |
| Outstanding Service-Show Host: | Christopher Lowell, The Christopher Lowell Show (DSC) |
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